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CBE Seminar - Nanofluidic Technologies For Cancer Treatment

Thursday, March 29, 2018
9:30am – 10:30am

Storrs Campus
UTEB 150

Nanofluidic Technologies For Cancer Treatment And Regenerative Medicine

Dr. James Lee Helen C. Kurtz Chair of the William G. Lowrie Department of Chemical & Biomolecular Engineering

Micro/Nanofluidics based biochips are a major platform technology being developed in our center for next-generation biomedical applications such as cancer cell interrogation and regenerative medicine. It requires strong interdisciplinary collaboration among engineering, science and medicine. Here we show the development of a new technology, nanochannel electroporation (NEP) and its applications in several frontier medical fields. Many transfection techniques can deliver biomolecules into cells, but the dose cannot be controlled precisely. Delivering well-defined amounts of materials into cells is important for various biological studies and therapeutic applications. NEP may deliver precise amounts of a variety of transfection agents into individual living cells. The cell to be transfected is positioned next to a nanochannel. Delivering a voltage pulse across the nanochannel produces an intense electric field over a very small area on the cell membrane, allowing a precise amount of transfection agent to be electrophoretically driven through the nanochannel, the cell membrane and into the cell cytoplasm, without affecting cell viability. Dose control is achieved by adjusting the duration and number of pulses. Both 2D and 3D NEP biochips are successfully used for living cancer stem cell interrogation, a unique methodology to analyze rare cells for preventing cancer metastasis and relapse, as well as non-viral generation of induced neurons (iNs), endothelial cells (iECs) and induced pluripotent stem cells (iPSCs) with high efficiency, an important step to realize regenerative medicine. A NEP patch is also developed for skin transfection activated regeneration. Using mouse models, we demonstrated the potential of NEP biochips and NEP patch for future clinical use in stroke recovery and wound healing.

Dr. Lee is the Helen C. Kurtz Professor of Chemical and Biomolecular Engineering at The Ohio State University (OSU). He founded and serves as the Director of NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymer Biomedical Devices (CANPBD) at OSU. He received a BS degree in chemical engineering from National Taiwan University and a Ph.D. degree in chemical engineering from University of Minnesota. Before joining OSU in 1982, he worked as a research scientist at General Tire and Rubber Company for 3 years. His research interest includes BioMEMS/NEMS, micro-/nanofabrication, and polymer and composite materials. He has more than 400 refereed journal publications, 30 patents and invention disclosures, and 14 book chapters. He was elected as the Fellow of Society of Plastics Engineers in 2001 and Fellow of American Institute for Medical and Biological Engineering in 2006. Dr. Lee received the 2008 Malcolm E. Pruitt Award from Council of Chemi

Contact:

leah.winterberger@uconn.edu

Chemical & Biomolecular Engineering (primary), Biomedical Engineering , Molecular and Cell Biology, UConn Health Biomedical Engineering, UConn Health Molecular Biology and Biophysics, UConn Master Calendar

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